The pulse beetle, Callosobruchus chinensis (Linnaeus, 1758), is a major stored pest of pulses (Fabaceae) in India. While effective control of this widespread and destructive insect pest is essential, continuous insecticide use poses environmental concerns, thereby necessitating alternative, eco-friendly control methods. This study evaluated the oviposition-deterrent effects of 7 synthetic compounds (linalool, guaiene, citral, estragole, 4-nonanone, alloaromadendrene, and citronellal) on C. chinensis. Linalool showed the strongest suppression of egg-laying at higher concentrations, with the lowest egg count at 500 ppm compared to the control, followed by citral, citronellal, and guaiene. L...

Cytogenetic stability is essential for the survival of micropropagated plants. While micropropagation allows rapid propagation of plants with desirable phenotypes, it may also produce genetic variations that are, at times, cytogenetically observable. Factors affecting these variations are not fully understood, and micropropagation protocols are optimized to produce genetically stable regenerants. The genetic stability of micropropagated plants is traditionally assessed by comparing the phenomorphological traits of the regenerants to the mother plant. However, genetic variation does not often result in obvious morphological changes. Therefore, molecular and cytogenetic markers are typicall...

Recent developments in rice cultivation in the Philippines are integrating sustainable methods in production systems to boost productivity and safeguard environmental integrity. An example of this is the use of biofertilizers such as Nutrio®, a microbial-based foliar fertilizer that promotes nitrogen fixation and the production of plant growth-promoting hormones. Nutrio® has been tested for sugarcane and is being explored for rice cultivation applications to maximize its utilization and benefits. This study aimed to assess the effects of Nutrio® biofertilizer on rice production in selected farms during the 2019 wet season and the 2020 dry season in Victoria, Laguna, Philippines. Six diffe...

Aloe vera is a perennial, succulent plant which has been used in folk medicine for wound-healing, as its roots have been reported to exhibit remarkable antimicrobial properties. However, collecting the roots is detrimental to plant growth and development. Hence, an alternative system of producing A. vera roots must be developed. Tissue culture is a potential tool to obtain A. vera root biomass, which can serve as raw materials for the large-scale production of antimicrobial compounds. In this study, individual shoots taken from the multiple shoot culture of A. vera were induced to form roots in MS medium supplemented with 1 μM of naphthaleneacetic acid (NAA), indolebutyric acid (IBA), and...

Lettuce is one of the most consumed fresh produce items in the country, but it also serves as a vehicle for human pathogens, posing a risk of foodborne illness. This study used conventional microbiological and molecular analyses to obtain the concentration and prevalence of Salmonella enterica in fresh Green Ice and Iceberg lettuce leaves from selected supermarkets in Los Baños, Laguna, Philippines. The efficacy of different household washing procedures in reducing S. enterica on lettuce leaves was also investigated. Of the 19 composite Green Ice lettuce and 21 composite Iceberg lettuce samples tested, 5.26% and 4.76%, respectively, were contaminated with S. enterica, both with a 3.6 MPN ...