The effect of the combination treatment of 1% chitosan coating and 3-d low temperature conditioning (LTC) at 10 °C was evaluated in maintaining the quality of red-fleshed dragon fruit during storage at 8 °C. Fruit subjected to LTC and chitosan coating treatments had slower respiration rate than the control. Ascorbic acid (AAC) and total phenolic contents (TPC) of the fruit subjected to the treatments were unaffected during storage. However, the decline in AAC after transfer to 20 °C was slower in treated fruit. Furthermore, the increase in flesh concentration of 1-aminocyclopropane-1-carboxylic acid (ACC) during storage at 8 °C was due to the inhibition of ethylene biosynthesis. The increase in electrolyte leakage, on the other hand, was a manifestation of cell breakdown as a result of chilling injury.