The study investigated the effect of wine waste extracts on antiproliferative property. Wine wastes were extracted using acetone/methylene chloride (A+M) and methanol (MeOH) and then fractionated using n-hexane, 85% aq. methanol (MeOH), butanol (BuOH) and distilled water. The cytotoxic activity of the wine wastes against AGS human gastric, HT-29 human colon and HT-1080 fibroblast cancer cell lines was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. All the extracts and fractions from the wine wastes decreased the growth of AGS cells, and the effect was concentration-dependent. The MeOH extract showed significantly higher inhibition against the growth of AGS cells compared with the A+M extract (P<0.05). The same trend was observed for the n-Hexane, 85% aq. MeOH, n-BuOH and water fractions. Among the fractions, the 85% aq. MeOH fraction showed the highest effect of 68% inhibition at the lowest concentration (0.025 mg mL^-1). In the HT-29 cancer cells, the pattern of growth inhibition by the crude extracts was a little different from that observed for the AGS cancer cells, with the A+M extract showing a higher effect (P<0.05). The n-BuOH and 85% aq. MeOH fractions were the most effective against the proliferation of HT-29 cancer cell lines (P<0.05). The pattern of growth inhibition in the HT-1080 cells was similar to that observed in the HT-29 cancer cells, with the A+M extract being the most effective. In addition, similar to the trend observed in the HT-29 cells, the 85% aq. MeOH fraction showed the highest inhibition of the growth of the HT-1080 cancer cells. Thus, the 85% aq. MeOH fraction from wine waste extracts would contain bioactive compounds such as polyphenols and flavonoids. There is a need for further research to separate and isolate these important compounds from the extracts.