The study aimed to characterize and establish baseline data on the quantity and quality of fresh avian semen utilizing a sample of Philippine native roosters. Twenty-two heads each were acquired from breeds Banaba, Joloano, and Paraoakan. The baseline data analysis revealed significant variations in semen volume among different genetic groups, with Banaba displaying the highest volume, followed by Joloano and Paraoakan. Banaba also exhibited the highest semen pH value of 7.27 when compared to the other two genetic groups (p < 0.05). Across various genetic groups of native chickens, the majority of semen samples demonstrated a thick and creamy consistency and color (42.6%), followed by thin and creamy (32.9%), with a statistical significance of p < 0.05. Furthermore, variations in sperm concentration were observed among genetic groups, with Paraoakan having the highest sperm concentration, followed by Banaba and Joloano (p < 0.05). When the semen from Banaba and Joloano native roosters were extended in Ringer’s solution, it exhibited good motility, with more than 50% of spermatozoa in motion, even after six hours of extension. In contrast, the pooled extended semen from Paraoakan native roosters displayed poor motility, particularly after eight hours of extension (p < 0.05). The study also showed that storage temperatures did not significantly affect all the sperm quality parameters of Banaba and Joloano. However, for Paraoakan, samples stored at low temperatures of 5 – 10 °C had significantly higher percent motile and progressive sperm, and lower percent static sperm than those stored at room temperature (22 – 25 °C) (p < 0.05).